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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in several tumors, which results in chromosome instability, and thus might lead to tumorigenesis. This protein was found to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important job in advertising mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are described. Two supplemental splice variants were noted but in depth nucleotide sequence information is not really still accessible.
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Blocking peptides are peptides that bind precisely for the target antibody and block antibody binding. These peptide normally has the epitope recognized from the antibody.
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This guide outlines further more probable optimization of mobile and immunoassay parameters to make sure the absolute best final results are...
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This manual outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody versus the antibody alone, you can see which staining is precise.
Goal Details Cyclin E belongs to the highly conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit unique expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. website This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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